GLP-1 Localisation and Proglucagon Gene Expression in Healthy and Diabetic Mouse Ileum
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Published on: April 14, 2010
Serap Koral Taşçı1, Seyit Ali Bingöl2
1Department of Histology and Embryology, Faculty of Veterinary Medicine, Kafkas University, 36100 Kars, Turkey.
Introduction:
Glucagon-like peptide-1 (GLP-1) is a polypeptide that is mainly produced by intestinal L cells and is encoded by the proglucagon gene. In this study, GLP-1 localisation was investigated in the ileum of healthy and diabetic mice by immunohistochemistry and proglucagon gene expression was assayed by reverse transcription-polymerase chain reaction.
Material And Methods:
This study included 18 male Balb/c mice that were divided into diabetic, sham, and control groups. Mice in the diabetic group received 100 mg/kg of streptozotocin. Immunohistochemical expression of GLP-1 was determined using the avidin-biotin-peroxidase complex technique, and proglucagon gene expression was determined by RT-PCR.
Results:
Analysis of GLP-1 immunohistochemical localisation showed that GLP-1-immunopositive cells (L cells) were present between epithelial cells in the intestinal crypts. The intensity and localisation of GLP-1 immunoreactivity were similar among the mice in all the groups. Proglucagon gene expression levels were also statistically similar among the mice in all the groups.
Conclusion:
No difference was demonstrated among the mice in the diabetic, sham, or control groups with respect to proglucagon gene expression and GLP-1 localisation in the ileum, suggesting that diabetes does not affect proglucagon gene expression in the ileum.
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