Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

8.7K
Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
8.7K
Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

2.7K
2.7K
Mutations01:39

Mutations

94.5K
Overview
94.5K
Mutations01:35

Mutations

44.5K
Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
44.5K
Viral Mutations00:36

Viral Mutations

39.9K
A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
39.9K
Allosteric Regulation01:08

Allosteric Regulation

63.3K
Allosteric regulation of enzymes occurs when the binding of an effector molecule to a site that is different from the active site causes a change in the enzymatic activity. This alternate site is called an allosteric site, and an enzyme can contain more than one of these sites. Allosteric regulation can either be positive or negative, resulting in an increase or decrease in enzyme activity. Most enzymes that display allosteric regulation are metabolic enzymes involved in the degradation or...
63.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Physics Determines and Evolution Shapes Basic Units of Protein Function and Allosteric Regulation.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

Antigen-directed single domain antibody-based TNFR1 agonists elicit preferential killing of HER2-overexpressing cancer cells.

iScience·2026
Same author

From Extreme Environments in Nature to Molecular and Cellular Adaptation and Functional Regulation.

Journal of molecular biology·2026
Same author

Multifunctional antibody-derived IL-12 mimetics: prospects of future clinical development.

Expert opinion on investigational drugs·2026
Same author

On the Relationship Between Protein Stability, Thermostability, and Allosteric Signaling.

Journal of molecular biology·2025
Same author

From discovery to the clinic: structural insights, engineering options, clinical, and 'next wave' applications of camelid-derived single-domain antibodies.

mAbs·2025

Related Experiment Video

Updated: Feb 3, 2026

Comparing Bibliometric Analysis Using PubMed, Scopus, and Web of Science Databases
05:02

Comparing Bibliometric Analysis Using PubMed, Scopus, and Web of Science Databases

Published on: October 24, 2019

33.7K

AlloMAPS: allosteric mutation analysis and polymorphism of signaling database.

Zhen Wah Tan1, Wei-Ven Tee1,2, Enrico Guarnera1

  • 1Bioinformatics Institute, Agency for Science, Technology and Research (A*STAR), 30 Biopolis Street, #07-01, Matrix, 138671 Singapore.

Nucleic Acids Research
|October 27, 2018
PubMed
Summary

The AlloMAPS database offers insights into allosteric communication and energetics using a structure-based statistical mechanical model of allostery (SBSMMA). This resource aids in identifying regulatory sites and designing allosteric effectors for proteins.

More Related Videos

The ITS2 Database
16:17

The ITS2 Database

Published on: March 12, 2012

32.3K
Generation of Comprehensive Thoracic Oncology Database - Tool for Translational Research
11:18

Generation of Comprehensive Thoracic Oncology Database - Tool for Translational Research

Published on: January 22, 2011

16.5K

Related Experiment Videos

Last Updated: Feb 3, 2026

Comparing Bibliometric Analysis Using PubMed, Scopus, and Web of Science Databases
05:02

Comparing Bibliometric Analysis Using PubMed, Scopus, and Web of Science Databases

Published on: October 24, 2019

33.7K
The ITS2 Database
16:17

The ITS2 Database

Published on: March 12, 2012

32.3K
Generation of Comprehensive Thoracic Oncology Database - Tool for Translational Research
11:18

Generation of Comprehensive Thoracic Oncology Database - Tool for Translational Research

Published on: January 22, 2011

16.5K

Area of Science:

  • Biochemistry
  • Structural Biology
  • Computational Biology

Background:

  • Allosteric communication is crucial for protein function and regulation.
  • Understanding allosteric signaling aids in drug discovery and protein design.
  • Existing databases lack comprehensive data on allosteric energetics and causality.

Purpose of the Study:

  • To introduce the AlloMAPS database, a novel resource for exploring allosteric communication.
  • To provide data on the causality and energetics of allosteric signaling using the structure-based statistical mechanical model of allostery (SBSMMA).
  • To facilitate the evaluation of allosteric effects in identifying regulatory sites and designing allosteric modulators.

Main Methods:

  • The structure-based statistical mechanical model of allostery (SBSMMA) was employed to generate data.
  • The AlloMAPS database integrates data from three protein sets: annotated functional sites, PDBselect chains, and proteins with pathological SNPs.
  • Allosteric Signaling Maps (ASMs) were computed via exhaustive scanning for stabilizing/destabilizing mutations and modulation ranges.

Main Results:

  • The AlloMAPS database contains comprehensive data on allosteric signaling energetics and causality for diverse protein sets.
  • It enables exploration of allosteric modulation by ligand binding, SNPs, and user-defined mutations.
  • Allosteric Signaling Maps (ASMs) provide detailed insights into sequence position-dependent allosteric effects.

Conclusions:

  • The AlloMAPS database is a valuable resource for studying protein allostery.
  • It supports the identification of novel allosteric sites and the design of allosteric drugs.
  • The database advances our understanding of allosteric communication and its implications in biological systems.