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Related Experiment Video

Updated: Feb 3, 2026

Thermochemical Studies of NiII and ZnII Ternary Complexes Using Ion Mobility-Mass Spectrometry
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Three-Fragment Fluorescence Complementation for Imaging of Ternary Complexes under Physiological Conditions.

Minghai Chen1,2, Wei Li1, Zhi-Ping Zhang1

  • 1State Key Laboratory of Virology, Wuhan Institute of Virology , Chinese Academy of Sciences , Wuhan 430071 , China.

Analytical Chemistry
|October 27, 2018
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Summary

We developed a three-fragment fluorescence complementation (TFFC) system to visualize protein-protein interactions in living cells. This method successfully identified weak-affinity ternary complexes and aided HIV-1 integration analysis.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Protein-protein interactions (PPIs) are crucial for cellular processes.
  • Identifying multiprotein complexes, especially ternary complexes, in living cells under physiological conditions is challenging.
  • Understanding PPIs is key to analyzing biological pathways and molecular mechanisms.

Purpose of the Study:

  • To develop a novel method for visualizing and identifying ternary protein complexes in living cells.
  • To apply this method to study complex biological processes like HIV-1 integration.

Main Methods:

  • Developed a three-fragment fluorescence complementation (TFFC) system using a split Venus fluorescent protein.
  • Applied the TFFC system to visualize weak-affinity ternary complexes in living cells.
  • Utilized the TFFC system to analyze multi-interactions during HIV-1 integration.

Main Results:

  • Successfully visualized ternary complexes in living cells under physiological conditions.
  • Identified multi-interactions involving weak-affinity ternary complexes.
  • Revealed the role of the barrier-to-autointegration factor protein in HIV-1 integration.

Conclusions:

  • The Venus-based TFFC system is an effective tool for visualizing and identifying ternary complexes in living cells.
  • This TFFC system advances the study of complex molecular interactions and biological pathways.
  • The TFFC system provides new insights into the mechanism of HIV-1 integration.