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Urease activity according to the different inhibitors
Mateus P Gomes1, Everaldo Zonta2, Juliano B Stafanato2
1Departamento de Solos, Universidade Federal de Viçosa, Avenida Peter Henry Rolfs, s/n, Campus Universitário, 36570-900 Viçosa, MG, Brazil.
Anais Da Academia Brasileira De Ciencias
|October 27, 2018
Summary
N-butyl thiophosphoric triamide (NBPT) effectively inhibits urease activity at low concentrations. Humic acid showed inhibitory effects after 36 hours, while zeolite had no significant impact on urease activity.
Area of Science:
- Agronomy
- Soil Science
- Biochemistry
Background:
- Urease enzyme activity significantly impacts nitrogen fertilizer efficiency.
- Inhibitors are crucial for mitigating nitrogen loss in agricultural soils.
- NBPT, humic acid, and zeolite are potential urease inhibitors.
Purpose of the Study:
- To evaluate the efficacy of NBPT, humic acid, and zeolite as urease inhibitors.
- To determine the optimal concentrations for inhibiting urease activity.
- To compare the effectiveness of different urease inhibitors.
Main Methods:
- Two experiments were conducted using a completely randomized design in split plots.
- Urease activity was quantified using methods by May and Douglas (1976) and Witte and Medina-Escobar (2001).
- Fifteen fertilizers with varying concentrations of NBPT, humic acid, and zeolite were analyzed.
Main Results:
- NBPT demonstrated significant urease inhibition, with 0.02% (m/m) being sufficient, regardless of concentration.
- Humic acid reduced urease activity after 36 hours when analyzed by the May and Douglas method.
- Zeolite did not significantly affect urease activity under the May and Douglas method conditions.
Conclusions:
- NBPT is a highly effective urease inhibitor, even at low concentrations.
- Humic acid exhibits delayed urease inhibitory effects.
- Zeolite showed no significant urease inhibition in this study.
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