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Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions
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Rapid detection of Yersinia enterocolitica serotype O:3 using a duplex PCR assay

Leonardo Alves Rusak1, Rodrigo de Castro Lisboa Pereira2, Isabelle Geoffroy Freitag3

  • 1Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Pesquisa em Infecção Hospitalar, Rio de Janeiro /RJ, Brazil; Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Zoonoses Bacterianas/Setor Listeria, Rio de Janeiro /RJ, Brazil.

Journal of Microbiological Methods
|October 27, 2018
PubMed

Insights

A new duplex PCR assay effectively detects Yersinia enterocolitica ssp. palearctica biotype 4/O:3, offering a faster and more accurate method than traditional cultures for public health surveillance.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Public Health

Background:

  • Yersinia enterocolitica is a zoonotic bacterium causing human gastrointestinal and extraintestinal diseases.
  • Y. enterocolitica ssp. palearctica biotype 4/O:3 is a significant public health concern in Europe.
  • Traditional culture-based methods for Y. enterocolitica detection are often unsuccessful.

Purpose of the Study:

  • To develop a novel duplex PCR assay for rapid and accurate detection of Y. enterocolitica ssp. palearctica biotype 4/O:3.
  • To overcome limitations of conventional culture-based diagnostic methods.

Main Methods:

  • A single duplex PCR assay was developed combining primers for tufA and rfbC genes.
  • The assay was validated using 88 Yersinia strains and DNA from stool samples of HIV-positive and HIV-negative pregnant women.

Main Results:

  • The duplex PCR assay demonstrated high efficacy in detecting Y. enterocolitica ssp. palearctica biotype 4/O:3.
  • The assay was 16 times more effective than culture-based methods for detecting Yersinia spp. in stool samples.
  • The method also detected other Y. enterocolitica serotypes and Yersinia species.

Conclusions:

  • The developed duplex PCR assay is a rapid and reliable tool for Yersinia detection.
  • This assay can significantly aid hospital and veterinary surveillance programs for Yersinia worldwide.
Keywords:
DetectionPCRScreening methodYersinia enterocolitica O:3Yersinia spp

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