Draft genome sequence of a methicillin-resistant Staphylococcus epidermidis isolate from swine

Area of Science:

• Microbiology
• Genomics
• Veterinary Science

Background:

• Methicillin-resistant Staphylococcus epidermidis (MRSE) is a significant pathogen in both human and animal health.
• Understanding the genetic makeup of MRSE strains in livestock is crucial for surveillance and control.
• Staphylococcus epidermidis strains from pigs can potentially serve as reservoirs for antimicrobial resistance genes.

Purpose of the Study:

• To report the draft genome sequence of a methicillin-resistant Staphylococcus epidermidis strain isolated from pigs in South Africa.
• To identify antimicrobial resistance genes, plasmids, and sequence types within this strain.
• To contribute to the understanding of the genetic diversity of S. epidermidis in African livestock.

Main Methods:

• Whole-genome sequencing was performed using the Illumina MiSeq platform.
• De novo assembly of genomic reads was conducted using CLC Genomics Workbench.
• Bioinformatic analysis included annotation, identification of antimicrobial resistance genes, plasmids, and sequence typing.

Main Results:

• A draft genome sequence of Staphylococcus epidermidis strain PR246B0 (sequence type 59) was obtained.
• The genome contains a 2,537,769 bp circular chromosome with 32.32% G-C content.
• Antimicrobial resistance genes conferring resistance to β-lactams, fluoroquinolones, aminoglycosides, fosfomycin, macrolides, lincosamides, and tetracycline were identified.
• Seven plasmid replicon types were detected.

Conclusions:

• The reported genome sequence provides valuable insights into the genetic architecture of S. epidermidis in Africa.
• This genomic data can aid in future epidemiological studies and antimicrobial resistance surveillance in livestock.
• The findings highlight the importance of monitoring MRSE in animal populations for public health implications.