Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

13.5K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
13.5K
Atomic Force Microscopy01:08

Atomic Force Microscopy

4.5K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
4.5K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

16.3K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
16.3K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

1.3K
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
1.3K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

21.1K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.1K
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

14.6K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
14.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Light-driven spatial proteomics: Photocatalytic strategies for mapping protein microenvironments.

Current opinion in chemical biology·2026
Same author

TRIPODD Enables Single-Cell Quantification of Therapeutic Efficacy.

Molecular pharmaceutics·2026
Same author

Considerations for the use of targeted fluorescence contrast agents to detect circulating cancer cell populations with diffuse <i>in vivo</i> flow cytometry.

Journal of biomedical optics·2026
Same author

Reflect: reporting guidelines for preclinical, translational and clinical fluorescence molecular imaging studies.

Npj imaging·2025
Same author

Circulating Neoplastic-Immune Hybrid Cells Are Biomarkers of Occult Metastasis and Treatment Response in Pancreatic Cancer.

Cancers·2024
Same author

Two-color fluorescence-guided surgery for head and neck cancer resections.

Journal of biomedical optics·2024

Related Experiment Video

Updated: Feb 3, 2026

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
07:49

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Published on: June 8, 2020

8.8K

Superresolution microscopy with novel BODIPY-based fluorophores.

Amy M Bittel1, Isaac S Saldivar1, Nick J Dolman2

  • 1Biomedical Engineering Department, Oregon Health & Science University, Portland, Oregon, United States of America.

Plos One
|October 27, 2018
PubMed
Summary
This summary is machine-generated.

Researchers optimized imaging buffers for BODIPY fluorophores, enabling multicolor single-molecule localization microscopy (SMLM). This advancement expands color options for SMLM by combining novel BODIPY probes with existing photoswitchable dyes.

More Related Videos

Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy
07:31

Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy

Published on: August 19, 2021

2.8K
Intravital Subcellular Microscopy of the Mammary Gland
04:14

Intravital Subcellular Microscopy of the Mammary Gland

Published on: June 24, 2022

1.4K

Related Experiment Videos

Last Updated: Feb 3, 2026

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
07:49

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Published on: June 8, 2020

8.8K
Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy
07:31

Measuring the pH, Redox Chemistries, and Degradative Capacity of Macropinosomes using Dual-Fluorophore Ratiometric Microscopy

Published on: August 19, 2021

2.8K
Intravital Subcellular Microscopy of the Mammary Gland
04:14

Intravital Subcellular Microscopy of the Mammary Gland

Published on: June 24, 2022

1.4K

Area of Science:

  • Biophysics
  • Microscopy
  • Molecular Biology

Background:

  • Multicolor single-molecule localization microscopy (SMLM) offers nanoscale resolution (~10-20 nm) for studying biomolecular interactions.
  • Effective multicolor SMLM relies on fluorophores with minimal spectral crosstalk and compatible photoswitching conditions.
  • Existing photoswitchable fluorophores often have limited Stokes shifts (<30 nm), restricting the number of resolvable colors.

Purpose of the Study:

  • To identify optimal imaging buffer conditions for BODIPY-based fluorophores for multicolor SMLM.
  • To expand the palette of available probes for multicolor SMLM by developing novel BODIPY fluorophores.
  • To overcome limitations of existing fluorophores with short Stokes shifts.

Main Methods:

  • Screened 35 imaging buffer conditions, testing seven redox reagents and five additives.
  • Evaluated compatibility of buffer conditions with BODIPY-based fluorophores for photoswitching.
  • Developed and characterized novel photoswitchable BODIPY fluorophores with varied Stokes shifts.

Main Results:

  • Identified compatible imaging buffer combinations for BODIPY fluorophores.
  • Demonstrated the utility of novel BODIPY fluorophores with longer Stokes shifts for SMLM.
  • Successfully combined new BODIPY probes with commercial fluorophores for multicolor SMLM.

Conclusions:

  • Optimized buffer conditions facilitate the routine use of BODIPY fluorophores in multicolor SMLM.
  • Novel BODIPY fluorophores provide valuable additional color options for advanced SMLM applications.
  • This work enhances the capabilities of SMLM for visualizing complex biological systems.