Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Amplifiable expression vectors for mammalian cell lines.

N Israël, N Chenciner, R E Streeck

    Gene
    |January 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Deeply Virtual Compton Scattering Cross Section at High Bjorken x_{B}.

    Physical review letters·2022
    Same author

    Form Factors and Two-Photon Exchange in High-Energy Elastic Electron-Proton Scattering.

    Physical review letters·2022
    Same author

    Deep Exclusive Electroproduction of π^{0} at High Q^{2} in the Quark Valence Regime.

    Physical review letters·2021
    Same author

    Alpha-fetoprotein in the early neonatal period--a large study and review of the literature.

    Clinica chimica acta; international journal of clinical chemistry·2004
    Same author

    The role of umbilical cord alpha fetoprotein as a screening tool for neonatal hyperbilirubinemia.

    American journal of perinatology·2004
    Same author

    The infiltration kinetics of simian immunodeficiency virus-specific T cells drawn to sites of high antigenic stimulation determines local in vivo viral escape.

    Proceedings of the National Academy of Sciences of the United States of America·2001
    Same journal

    TBX6 promotes proliferation, invasion, and migration in colorectal cancer: Integrated transcriptomic and protein interaction network analysis.

    Gene·2026
    Same journal

    Face/off: phase-specific modeling of lineage plasticity using near-patient models in genitourinary cancers.

    Gene·2026
    Same journal

    Hierarchical analysis of metabolic phenotype reveals distinct microbiota and circulatory transcriptome in metabolic dysfunction-associated steatotic liver disease.

    Gene·2026
    Same journal

    Mutation T71R enhanced the structural stability and functional activity of wild type superoxide dismutase cloned from soil metagenome.

    Gene·2026
    Same journal

    Reduced ATXN1 expression as an adverse prognostic indicator in Acute myeloid leukemia.

    Gene·2026
    Same journal

    Constructing regulatory networks of Rubisco post-translational modifications: a novel avenue for engineering environment adaptive plants.

    Gene·2026
    See all related articles

    This study developed a versatile mammalian expression vector for high-level gene expression and amplification. The vector, utilizing the human metallothionein IIA promoter, achieved significant and stable expression of the Hepatitis B virus (HBV) surface antigen gene.

    Area of Science:

    • Molecular Biology
    • Gene Expression
    • Mammalian Cell Culture

    Background:

    • Development of efficient expression vectors is crucial for molecular biology research and biotechnology.
    • Mammalian cell systems are widely used for producing recombinant proteins due to their post-translational modification capabilities.

    Purpose of the Study:

    • To construct and evaluate a prototype general expression vector for mammalian cells.
    • To assess the vector's efficiency in driving gene expression and enabling gene amplification.
    • To optimize expression of the Hepatitis B virus (HBV) surface antigen gene.

    Main Methods:

    • Construction of a novel mammalian expression vector containing a strong, broadly active promoter and a selectable marker for gene amplification.
    • Evaluation of vector performance using the Hepatitis B virus (HBV) S gene encoding the surface antigen.

    Related Experiment Videos

  • Utilized the human metallothionein IIA promoter for high-level gene expression in mouse L and monkey Vero cells.
  • Employed a promoterless thymidine kinase (tk) gene for gene amplification in mouse Ltk- cells via selection in hypoxanthine, aminopterine, and thymidine (HAT) medium.

    • Main Results:

    • The human metallothionein IIA promoter demonstrated high efficiency for S gene expression in both mouse L and monkey Vero cells.
    • Inclusion of the promoterless tk gene facilitated gene amplification in Ltk- cells, leading to 50-100 fold increases in S gene expression.
    • While some amplified clones were unstable, several maintained high expression levels for over a year.

    Conclusions:

    • The developed expression vector is a promising tool for achieving high-level and amplified gene expression in mammalian cells.
    • The human metallothionein IIA promoter is a potent element for driving gene expression in diverse mammalian cell types.
    • The vector system offers a viable strategy for stable, long-term production of recombinant proteins.