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Interaction and fusion dynamics between cellular blebs.

Chao Fang1, Tsz Hin Hui1, Xi Wei1

  • 1Department of Mechanical Engineering, The University of Hong Kong, Hong Kong; HKU-Shenzhen Institute of Research and Innovation (HKU-SIRI), Shenzhen, Guangdong, China.

Journal of Biomechanics
|October 28, 2018
PubMed
Summary
This summary is machine-generated.

Cellular membrane blebbing dynamics are influenced by neighboring blebs and fusion. This study quantifies how bleb growth, retraction, and coalescence depend on factors like pressure, time lag, and membrane-cortex adhesion.

Keywords:
Cell adhesionCell blebbingMembrane deformation

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Area of Science:

  • Cell Biology
  • Biophysics
  • Computational Biology

Background:

  • Membrane blebbing is crucial for cellular processes like migration and apoptosis.
  • The interaction and fusion dynamics of neighboring cellular blebs are not well understood.

Purpose of the Study:

  • To investigate how neighboring blebs influence each other's growth and retraction.
  • To examine the fusion dynamics between adjacent cellular blebs.
  • To develop a model predicting bleb behavior based on physical factors.

Main Methods:

  • Developed a boundary integral model to simulate cell membrane shape evolution during blebbing.
  • Combined theoretical modeling with experimental observations.
  • Analyzed the impact of intracellular pressure, time lag, and membrane-cortex adhesion.

Main Results:

  • Bleb formation induces intracellular pressure drops, affecting subsequent bleb growth.
  • The volume of a second bleb is dependent on its initial size and temporal relationship to the first bleb.
  • Increased membrane-cortex adhesion transitions bleb coalescence from smooth to abrupt, or prevents fusion entirely.
  • Phase diagrams illustrate transitions in bleb fusion based on pressure and separation.

Conclusions:

  • Neighboring blebs significantly influence each other's dynamics through pressure changes and fusion.
  • Physical parameters like adhesion strength, pressure, and separation dictate bleb interaction and coalescence.
  • The study provides quantitative predictions for bleb behavior, validated by experiments.