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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
Published on: March 20, 2015
Thin layer chromatography combined with surface-enhanced raman spectroscopy for rapid sensing aflatoxins
Lu-Lu Qu1, Qin Jia1, Chunyuan Liu1
1School of Chemistry and Materials Science, Jiangsu Normal University, Xuzhou, Jiangsu, 221116, China.
Abstract:
A fast and facile method was developed for on-site detection of aflatoxins (AFs) in moldy agricultural products using thin layer chromatography combined with surface-enhanced Raman spectroscopy (TLC-SERS). Four different AFs were successfully separated by TLC and then a small portable Raman spectrometer, with gold colloids as the SERS-active substrate, was applied to identify the separated spots. TLC-SERS application to on-site detection of AFs was systematically investigated. Qualitative and quantitative AF detection was found to be easily accomplished and limits of detection were estimated to be 1.5 × 10-6, 1.1 × 10-5, 1.2 × 10-6, and 6.0 × 10-7 M for AFB1, AFB2, AFG1, and AFG2, respectively. The proposed method was also highly selective, enabling successful AF identifications in complex extracts from moldy peanuts. The study showed that TLC-SERS could be effectively used for separation and detection of these four AFs, demonstrating good prospects for on-site qualitative screening of agricultural products.
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