Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Light Acquisition02:16

Light Acquisition

9.6K
In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
9.6K
Flow Sheet01:17

Flow Sheet

2.8K
Flowsheets are valuable tools in nursing documentation. They enable healthcare professionals to efficiently record and monitor various patient assessments and measurements in a consolidated format.
Here's a closer look at the examples of flowsheets commonly used by nurses:
Graphic Sheet Documentation:
2.8K
Characteristics of Practical Op Amps01:16

Characteristics of Practical Op Amps

864
A difference amplifier, a crucial component in numerous electronic devices, ideally amplifies only the difference-mode signal, which is the difference between two input signals. However, in practical circuits, the output voltage depends on both the differential gain and the common-mode gain.
The ratio of differential gain to the common-mode gain is defined as the common-mode rejection ratio (CMRR). This ratio quantifies the ability of operational amplifiers (op-amps) to reject common-mode...
864
Theoretical Foundations of Nursing Practice01:30

Theoretical Foundations of Nursing Practice

17.4K
Theories play an essential role in organizing patient care. Theories refer to a proposed or followed belief, policy, or procedure that is the basis for action. Nursing theories are knowledge-based concepts that guide nurses' actions, influence nursing education and practice, and allow nurses to care for their patients.
Theories provide a perspective to assess patients' conditions and organize data and methods. They also assist in analyzing and interpreting information. They represent a...
17.4K
Equivalent Circuits for Practical Transformers01:28

Equivalent Circuits for Practical Transformers

1.4K
The practical equivalent circuits of single-phase two-winding transformers exhibit significant deviations from their idealized versions due to the inherent properties of winding resistance and finite core permeability. These properties result in real and reactive power losses, affecting the transformer's performance. Understanding these deviations is crucial for designing more efficient transformers.
In a practical transformer, each winding exhibits resistance and leakage reactance. The...
1.4K
Light as Energy01:35

Light as Energy

95.9K
The energy required to carry out photosynthesis is light— typically electromagnetic radiation from the sun. The range of all possible wavelengths is known as the electromagnetic spectrum.
Photons
A photon is a discrete electromagnetic particle or bundle of energy. Photons are characterized by their frequency, wavelength, and amplitude, similar to the properties of a wave. Waves with higher frequencies transmit more energy and have shorter wavelengths than longer wavelengths that transmit...
95.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PCM1 coordinates centrosome asymmetry with polarized endosome dynamics to regulate daughter cell fate.

Nature communications·2025
Same author

Imaging cellular activity simultaneously across all organs of a vertebrate reveals body-wide circuits.

bioRxiv : the preprint server for biology·2025
Same author

inTRACKtive: a web-based tool for interactive cell tracking visualization.

Nature methods·2025
Same author

Ultrack: pushing the limits of cell tracking across biological scales.

Nature methods·2025
Same author

A modular chemigenetic calcium indicator for multiplexed in vivo functional imaging.

Nature methods·2024
Same author

Ultrack: pushing the limits of cell tracking across biological scales.

bioRxiv : the preprint server for biology·2024
Same journal

High-throughput measurements of protein domain functions using magnetic separation.

Nature protocols·2026
Same journal

Inducing physiological polarity and performing gene editing using CRISPR-Cas9 in human trophoblast organoids.

Nature protocols·2026
Same journal

Photocatalytic low-temperature defluorination of PTFE.

Nature protocols·2026
Same journal

Multimodal imaging and quantification of lanthanide chelate-labeled micro- and nanoplastics in plants.

Nature protocols·2026
Same journal

Facilitating structure-based drug discovery with an artificial intelligence-driven virtual screening platform.

Nature protocols·2026
Same journal

Yeast nuclei-mediated precise delivery of synthetic megabase-scale human DNA into mammalian embryos.

Nature protocols·2026
See all related articles

Related Experiment Video

Updated: Feb 3, 2026

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development
13:01

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

Published on: April 10, 2016

34.7K

A practical guide to adaptive light-sheet microscopy.

Loïc A Royer1, William C Lemon2, Raghav K Chhetri2

  • 1Chan Zuckerberg Biohub, San Francisco, CA, USA. loic.royer@czbiohub.org.

Nature Protocols
|October 28, 2018
PubMed
Summary
This summary is machine-generated.

Researchers can now optimize light-sheet microscopy with AutoPilot, an adaptive imaging framework. This system enhances spatial resolution and signal strength in living specimens, improving imaging quality for biological research.

More Related Videos

Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy
05:27

Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy

Published on: September 28, 2022

2.9K
Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish
07:28

Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish

Published on: February 27, 2014

19.5K

Related Experiment Videos

Last Updated: Feb 3, 2026

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development
13:01

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development

Published on: April 10, 2016

34.7K
Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy
05:27

Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy

Published on: September 28, 2022

2.9K
Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish
07:28

Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish

Published on: February 27, 2014

19.5K

Area of Science:

  • Microscopy
  • Biotechnology
  • Optical Engineering

Background:

  • Light-sheet microscopy is a powerful technique for imaging living organisms.
  • Optimizing spatial resolution and signal strength remains a challenge.
  • Mismatches in focal planes can degrade image quality.

Purpose of the Study:

  • To present an adaptive imaging framework, AutoPilot, for light-sheet microscopy.
  • To enable automated optimization of spatial resolution and signal strength.
  • To provide a protocol for implementing adaptive imaging in existing or new microscopes.

Main Methods:

  • Developed AutoPilot, a hardware and software framework for adaptive imaging.
  • Implemented modules for measuring and compensating focal plane mismatches.
  • Provided detailed protocols, technical drawings, and source code for implementation.

Main Results:

  • Near diffraction-limited performance achieved in multicellular organisms.
  • Resolution and signal strength improved two- to fivefold.
  • Successful implementation demonstrated in fruit fly and zebrafish embryos.

Conclusions:

  • AutoPilot offers a practical solution for enhancing light-sheet microscopy.
  • The framework is adaptable for both existing and new microscope setups.
  • This advancement significantly improves imaging capabilities for developmental biology and other fields.