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Pharmacokinetics and tissue distribution of oroxin B in rats using a validated LC-MS/MS assay
Kai Niu1, Huiyu Yan2, Chunjie Guo3
1Department of Otorhinolaryngology Head and Neck Surgery, The First Hospital of Jilin University, Changchun, China.
Abstract:
A highly sensitive LC-MS/MS method was developed to measure oroxin B in rat plasma and tissue homogenates. The analyte and IS were isolated from biological matrices by a simple protein precipitation followed by centrifugation. Detection was conducted by electrospray negative-ionization mass spectrometry in selected-reaction monitoring mode. The assay was linear in the concentration range 4.52-904 ng/mL with intra- and inter-day precision of <14.41%. It was successfully applied to the pharmacokinetics and tissue distribution studies of oroxin B after an intravenous dose of 1.0 mg/kg in rats. The results would be useful for further development of oroxin B.
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