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'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens
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Validation of Workflow Changes, Phage Concentration and Reformatted Detection Threshold for the Sample6 DETECT/L™

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  • 1Sample6, 12 Gill St, Suite 4200, Woburn, MA 01801.

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This summary is machine-generated.

The Sample6 DETECT/L assay for Listeria species detection on surfaces was modified to improve performance. Validation confirmed the enhanced assay meets or exceeds previous performance standards.

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Area of Science:

  • Food safety and microbiology
  • Environmental surface testing
  • Bacterial detection methods

Background:

  • The AOAC Research Institute certified Sample6 DETECT/L for Listeria species detection on stainless steel surfaces in 2014.
  • Modifications in 2016 enhanced reagents, phage concentration, sample volume, and compatibility with polyurethane sponges.

Purpose of the Study:

  • To validate workflow changes in the DETECT/L assay to enhance sensitivity and ease-of-use.
  • To ensure the modified assay continues to meet performance expectations.
  • To optimize assay performance through changes in phage concentration, detection threshold, and inclusion of a confirmation step.

Main Methods:

  • Inclusivity, exclusivity, and robustness testing were performed by Sample6.
  • A third-party laboratory compared the modified DETECT/L assay with the USDA reference method.
  • The study focused on stainless steel environmental surface matrices.

Main Results:

  • Validated changes included adjusted phage concentration, detection threshold, and the addition of a DETECT Check confirmation step.
  • The modified assay demonstrated enhanced accuracy and optimized performance.
  • Comparative study results indicated the changes meet or exceed the performance of the current DETECT/L configuration.

Conclusions:

  • The modifications to the Sample6 DETECT/L assay successfully enhanced its sensitivity, accuracy, and ease-of-use.
  • The updated assay maintains or improves upon its established performance for Listeria species detection.
  • The validated changes ensure the continued reliability of DETECT/L for environmental surface monitoring.