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Updated: Feb 3, 2026

Gold Nanoparticle Synthesis
Published on: July 10, 2021
Gold nanoparticles-based multifunctional nanoconjugates for highly sensitive and enzyme-free detection of E.coli K12
Yongjin Zou1, Jing Liang2, Zhe She3
1Guangxi Key Laboratory of Information Materials, Guilin University of Electronic Technology, Guilin 541004, PR China; Department of Physical and Environmental Sciences, University of Toronto Scarborough, Toronto M1C 1A4, Canada.
Abstract:
Immobilization of proteins on a biocompatible conductive interface is highly desirable for the fabrication of biosensors. In this study, a nanocomposite has been prepared by assembling well-distributed gold nanoparticles (AuNPs) on the surface of a polypyrrole-reduced graphene oxide (PPy-rGO) composite through electrostatic adsorption. This serves as a platform for immobilization of a capture antibody, which was conjugated onto the ferrocene doped polypyrrole-gold nanoparticles (PPy@Fc/AuNPs) composite. The design and performance of the biosensor was tested against detection of a whole-cell bacteria E. coli K12. This nanocomposite has a high surface area, good conductivity and biocompatibility, which is shown to be very suitable for enzyme-free detection of this bacteria. Results show excellent analytical performance with a linear range from 1.0 × 101 to 1.0 × 107 CFU mL-1 and a low detection limit of 10 CFU mL-1. The sensor has high selectivity, excellent reproducibility, and good stability.
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