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Network of three specific microRNAs influence type 2 diabetes through inducing insulin resistance in muscle cell

Maryam Honardoost1, Farid Keramati2, Ehsan Arefian3

  • 1Endocrine Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences, Tehran, Iran.

Journal of Cellular Biochemistry
|October 29, 2018
PubMed

Insights

Three specific microRNAs (miRNAs) were found to induce insulin resistance in muscle cells. Upregulation of these miRNAs impaired glucose uptake, increasing type 2 diabetes risk.

Area of Science:

  • Molecular Biology
  • Endocrinology
  • Genetics

Background:

  • Insulin resistance is a key predictor of type 2 diabetes.
  • MicroRNAs (miRNAs) are implicated as regulators of insulin resistance.

Purpose of the Study:

  • To investigate the role of miR-135, miR-202, and miR-214 in insulin resistance.
  • To assess the impact of these miRNAs on glucose uptake in muscle cell lines.

Main Methods:

  • Transfection of C2C12 and L6 muscle cells with selected miRNAs.
  • Measurement of glucose uptake levels.
  • Luciferase assay to evaluate miRNA target gene expression.

Main Results:

  • miRNA transfection significantly altered glucose uptake compared to controls.
  • miR-135, miR-202, and miR-214 targeted and downregulated Rho-associated coiled-coil containing protein kinase 1 and serine/threonine kinase 2.
  • Vesicle-associated membrane protein 2 was not significantly affected.
  • Upregulated miRNAs led to poor insulin stimulation of glucose uptake and an insulin-resistant phenotype.

Conclusions:

  • The studied miRNAs (miR-135, miR-202, miR-214) play a role in inducing insulin resistance.
  • These miRNAs contribute to the development of an insulin-resistant phenotype in muscle cells.
  • This finding suggests a potential link between these miRNAs and type 2 diabetes susceptibility.
Keywords:
insulin resitancemiR-135miR-202miR-214type 2 diabetes

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