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Hydroalcoholic Extract and Ethyl Acetate Fraction of Bixa orellana Leaves Decrease the Inflammatory Response to
José Lima Viana1, Adrielle Zagmignan1, Luís Felipe Lima Lobato1
1Pós-Graduação em Biologia Parasitária, Laboratório de Imunologia e Microbiologia das Infecções Respiratórias, Universidade Ceuma, São Luís, Brazil.
Evidence-Based Complementary and Alternative Medicine : Ecam
|October 30, 2018
Summary
The study shows that Bixa orellana extracts combat Mycobacterium abscessus infections. These natural extracts demonstrated antimicrobial and anti-inflammatory effects, offering potential for treating Mabs infections.
Area of Science:
- Pharmacology and Microbiology
- Natural Product Chemistry
Background:
- Rapidly growing mycobacteria (RGM), particularly Mycobacterium abscessus subsp. massiliense (Mabs), are causing increasing global infections.
- Severe Mabs infections lead to abscesses and significant inflammation.
Purpose of the Study:
- To evaluate the antimicrobial and anti-inflammatory properties of Bixa orellana leaf extracts against Mabs.
- To identify active compounds in the most effective Bixa orellana fraction.
Main Methods:
- Broth microdilution determined minimum inhibitory (MIC) and bactericidal (MBC) concentrations.
- Cytotoxicity assays used erythrocytes and RAW 264.7 cells.
- In vivo studies involved a Mabs-induced peritonitis mouse model and HPLC-MS analysis.
Main Results:
- Bixa orellana ethyl acetate fraction (BoEA) showed potent antimicrobial activity (MIC: 0.39 mg/mL, MBC: 6.25 mg/mL).
- Both hydroalcoholic extract (BoHE) and BoEA reduced nitric oxide production and inflammatory cell migration in vivo.
- No significant cytotoxicity or acute toxicity was observed.
Conclusions:
- Bixa orellana extracts possess significant antimicrobial and anti-inflammatory activities.
- The ethyl acetate fraction, rich in kaempferol-3-O-coumaroyl glucose, is particularly effective.
- Bixa orellana extracts show promise for treating Mabs infections.
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