Related Experiment Video

Updated: Feb 3, 2026

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma
12:03

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma

Published on: July 25, 2011

20.0K

Proteases are Modulated by Fascin in Oral Cancer Invasion

Min Kyeong Lee1, Ji Hyeon Park1, Seol Hwa Gi1

  • 1Department of Dental Hygiene, College of Health Science, Eulji University, Seongnam, Korea.

Journal of Cancer Prevention
|October 30, 2018
PubMed
Abstract

Insights

Fascin protein promotes cancer cell invasion by influencing protease activity. Depleting fascin significantly reduces cancer invasion, highlighting its role as a therapeutic target.

Area of Science:

  • Oncology
  • Cell Biology
  • Biochemistry

Background:

  • Cancer invasion is a key determinant of patient survival and prognosis.
  • Targeting invasion factors is crucial for effective cancer treatment.
  • Fascin is a protein implicated in cancer cell invasion.

Purpose of the Study:

  • To investigate the role of fascin in cancer invasion.
  • To determine how fascin influences cancer cell motility and protease activity.

Main Methods:

  • Established fascin-depleted cancer cell lines using lentiviral short hairpin RNA.
  • Assessed cancer invasion using Matrigel-Transwell assays and 3D culture systems.
  • Analyzed protease secretion and mRNA expression via antibody arrays and RT-qPCR.

Main Results:

  • Fascin expression was elevated in invasive cancer cells.
  • Fascin depletion led to reduced cancer invasion in vitro.
  • Inhibition of protease secretion and decreased intracellular protease mRNA levels were observed in fascin-depleted cells.

Conclusions:

  • Fascin is significantly involved in regulating protease activity and cancer cell invasion.
  • Fascin represents a strategic target for controlling cancer invasion and improving patient outcomes.
Keywords:
FascinGene silencingHead and neck neoplasmsMetalloproteasesTumor microenvironment

More Related Videos

Intraoperative Assessment of Resection Margins in Oral Cavity Cancer: This is the Way04:45

Intraoperative Assessment of Resection Margins in Oral Cavity Cancer: This is the Way

Published on: May 10, 2021

4.4K
Chick Heart Invasion Assay for Testing the Invasiveness of Cancer Cells and the Activity of Potentially Anti-invasive Compounds10:16

Chick Heart Invasion Assay for Testing the Invasiveness of Cancer Cells and the Activity of Potentially Anti-invasive Compounds

Published on: June 6, 2015

9.5K

Related Concept Videos

Oral Cavity01:11

Oral Cavity

The oral cavity, or the mouth, is a complex structure in humans that plays a vital role in our day-to-day lives. Its role is not only in chewing and swallowing food; it also plays a role in speech and facial expressions.
Teeth: The teeth are the hardest structures in our bodies. Humans have two sets of teeth throughout their lifetime: deciduous (baby) teeth and permanent teeth. Each tooth consists of several parts: the crown (visible part), the root (embedded in the jaw), enamel (hard outer...
3.1K
Oral Hypoglycemic Agents: Glinides01:06

Oral Hypoglycemic Agents: Glinides

Repaglinide (Prandin) and Nateglinide (Starlix), known as glinides, are oral insulin secretagogues that stimulate insulin release from pancreatic β cells by closing the ATP-sensitive potassium channels (KATP channel). Repaglinide controls insulin release from pancreatic β cells by managing potassium efflux. It shares two binding sites with sulfonylureas and also has a unique site, indicating overlapping mechanisms of action. With a rapid onset and a 4-7 hour duration, it effectively...
664
Psychosexual Stages of Personality: Oral01:16

Psychosexual Stages of Personality: Oral

The oral stage is the initial phase of Sigmund Freud's theory of psychosexual development, occurring from birth to approximately 12 to 18 months. During this period, the infant's mouth serves as the primary source of pleasure, with actions such as sucking, chewing, biting, and drinking playing a crucial role in reducing tension. These activities are essential not only for nourishment but also for the infant's psychological and emotional satisfaction.
Weaning, typically occurring...
3.1K
Assessing Body Temperature - Oral01:14

Assessing Body Temperature - Oral

Here are the steps to accurately measure oral temperature using an electronic thermometer:
Step 1:
Start by practicing proper hand hygiene to prevent the spread of microorganisms.
Step 2:
Take the thermometer out of the charging unit, switch it on, and wait for the ready sign.
Step 3:
Gently slide the probe cover until a click is heard. This simple action prevents cross-contamination and ensures the correct placement of the probe cover.
Step 4:
Instruct the patient to open their mouth and place...
1.6K
Oral Hypoglycemic Agents: Sulfonylureas01:17

Oral Hypoglycemic Agents: Sulfonylureas

Sulfonylureas are oral hypoglycemic agents utilized in treating type 2 diabetes. They are characterized by their unique sulfonylurea chemical structure. The family of sulfonylureas is divided into generations. First-generation sulfonylureas, including tolbutamide (Orinase), chlorpropamide (Diabinese), and tolazamide (Tolinase), trigger insulin release from pancreatic β cells and enhance peripheral tissues' insulin sensitivity. The second-generation members, such as glipizide...
1.1K
Non-Oral Extravascular Drug Absorption Routes01:15

Non-Oral Extravascular Drug Absorption Routes

Non-oral extravascular routes, which encompass sublingual, buccal, topical, intramuscular, and inhalation methods, primarily utilize passive diffusion to transport drugs into the systemic circulation. The absorption rates and effectiveness of these routes depend on the drug's physicochemical properties, as well as the patient's anatomical and pathophysiological state.
Lipophilic drugs that are stable at salivary pH (6) and exhibit minimal binding to the oral mucosa are absorbed more...
531