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Sendai Virus Propagation Using Chicken Eggs

Narihito Tatsumoto1, Moshe Arditi2,3, Michifumi Yamashita1

  • 1Department of Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, USA.

Bio-Protocol
|October 30, 2018
PubMed

Insights

Researchers developed an inexpensive chicken egg protocol to efficiently propagate Sendai virus (a paramyxovirus) for mouse studies. This method yields ample virus for research, including virotherapy and immunity investigations.

Area of Science:

  • Virology
  • Immunology
  • Animal Models

Background:

  • Sendai virus (paramyxovirus) is a negative-stranded RNA virus.
  • It causes pneumonia in mice but is not pathogenic to humans.
  • Efficient Sendai virus propagation is crucial for research in replication, immunity, virotherapy, and IgA nephropathy.

Purpose of the Study:

  • To describe a cost-effective protocol for propagating Sendai virus.
  • To enable large-scale virus generation for experimental use.

Main Methods:

  • Utilized chicken eggs for Sendai virus propagation.
  • Described a protocol for efficient virus generation.

Main Results:

  • The protocol enables the generation of a large quantity of Sendai virus.
  • The method is cost-effective.

Conclusions:

  • Chicken egg propagation provides an inexpensive and efficient way to produce Sendai virus.
  • This method supports extensive research applications, including animal and cell culture experiments.
Keywords:
Allantoic fluidChicken eggsHarvestMouse parainfluenza virusPropagationSendai virus

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