Developmental stages of the blue-breasted quail (Coturnix chinensis)

Chickens and Japanese quail (Coturnix japonica) have traditionally been the primary avian models in developmental biology research. Recently, the blue-breasted quail (Coturnix chinesis), the smallest species in the order Galliformes, has been proposed as an excellent candidate model in avian developmental studies owing to its precocious and prolific properties. However, data on the embryonic development of blue-breasted quail are scarce. Here, we developed a normal developmental series for the blue-breasted quail based on developmental features. The blue-breasted quail embryos take 17 days to reach the hatching period at 37.7°C. We documented specific periods of incubation in which significant development occurred, and created a 39-stage developmental series. The developmental series for the blue-breasted quail was almost identical to that for chickens and Japanese quail in the earlier stages of development (stages 1-16). Our staging series is especially useful at later stages of development (stages 34-39) of blue-breasted quail embryos as a major criterion of staging in this phase of development was the weight of embryos and the length of third toes.