Lethal Toxicity and Sublethal Metabolic Interference Effects of Sulfoxaflor on the Earthworm ( Eisenia fetida)

Testing for effects of pesticides on nontarget organisms is an integral part of ecological risk assessment. In the present study, the acute toxicity of sulfoxaflor to earthworms was evaluated using an artificial soil toxicity test, and sublethal effects were assessed through oxidative stress and metabolomics. Sulfoxaflor is a supertoxic pollutant to earthworms that easily bioaccumulates in earthworms, and contains LC₂, LC₁₀, and LC₅₀ values of 0.08 (0.04-0.13), 0.19 (0.11-0.25), and 0.54 (0.45-0.65) mg/kg, respectively. Sublethal doses of sulfoxaflor resulted in oxidative damage to earthworms in which antioxidant enzymatic activities including SOD, CAT, and GST were significantly inhibited and MDA content accumulated. Metabolomics analysis suggested that the energy metabolism and the urea cycle in earthworms were significantly activated, while nucleotide metabolism was depressed, which could cause DNA damage. The results suggest earthworms have the potential to be a new entry point for sulfoxaflor into the wildlife food chain. Since earthworms significantly contribute to soil function and ecosystems, the high safety risks of sulfoxaflor to the earthworm could extend to the environment. In view of these findings, more attention should be given to the risks sulfoxaflor poses to the environment through its effects on earthworms.