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Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors
Published on: January 20, 2023
Visual determination of malachite green in sea fish samples
Nataliya A Gavrilenko1, Tatyana N Volgina2, Evgeniy V Pugachev2
1Department of Analytical Chemistry, Tomsk State University, 634050 Tomsk, Russia.
Abstract:
Polymethylmethacrylate matrix has been developed as an analytical device for visual and spectrophotometric determination of malachite green. Based on the studied effects of different pH levels, temperature, contact time and diverse ions, optimum analysis conditions have been established. For malachite green, the calibration graph was linear in the range of 0.5-30 mg·L-1 in the samples with the detection limit of 1 mg·kg-1. The proposed simple method makes it possible to determine malachite green visually in the concentration range 8-30 mg·L-1 using a colour scale. The method has been implemented for determination of malachite green in various sea fish samples.
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