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Updated: Feb 3, 2026

Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
Published on: September 20, 2016
Lateral flow test for meat authentication with visual detection
Maria Magiati1, Vasiliki M Myridaki1, Theodore K Christopoulos2
1Department of Chemistry, University of Patras, Patras 26504, Greece.
Abstract:
A new lateral flow assay has been developed for meat authentication. The assay is based on gold nanoparticles and enables visual detection, by naked eye, of meat species-specific DNA sequences. The procedure includes DNA isolation from fresh meat samples, amplification of specific DNA sequences and detection by the lateral flow assay. The proposed assay is rapid and has been applied for the identification of four animal species: horse, pork, beef and sheep. The detection is completed within 25-30 min after amplification. The assay offers high detectability and good selectivity and reproducibility. As low as 0.01% of horse and 0.02% of pork DNA were detectable in binary mixtures by the reported lateral flow device.
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