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Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
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Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication

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Lateral flow test for meat authentication with visual detection.

Maria Magiati1, Vasiliki M Myridaki1, Theodore K Christopoulos2

  • 1Department of Chemistry, University of Patras, Patras 26504, Greece.

Food Chemistry
|October 31, 2018
PubMed
Summary

A novel lateral flow assay using gold nanoparticles allows for rapid, visual identification of meat species DNA. This DNA testing method accurately detects horse and pork DNA down to 0.01% in meat mixtures.

Keywords:
AdulterationBiosensorDNAGold nanoparticlesHorseMeatPorkStrip

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Area of Science:

  • Food Science and Technology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Accurate meat authentication is crucial for food safety, regulatory compliance, and consumer protection.
  • Traditional methods for meat species identification can be time-consuming and require specialized laboratory equipment.
  • There is a need for rapid, sensitive, and accessible methods for on-site meat species analysis.

Purpose of the Study:

  • To develop and validate a novel lateral flow assay for the visual detection of meat species-specific DNA.
  • To assess the assay's speed, sensitivity, selectivity, and reproducibility for identifying common meat species.
  • To evaluate the assay's performance in detecting low percentages of foreign DNA in meat mixtures.

Main Methods:

  • Development of a lateral flow assay utilizing gold nanoparticles for DNA detection.
  • Procedure involves DNA isolation from fresh meat, amplification of species-specific DNA sequences, and visual detection via the lateral flow device.
  • The assay was tested for the identification of horse, pork, beef, and sheep DNA.

Main Results:

  • The developed lateral flow assay provides visual detection of meat species-specific DNA within 25-30 minutes post-amplification.
  • The assay demonstrated high detectability, good selectivity, and reproducibility across tested meat species.
  • The assay successfully detected as low as 0.01% horse DNA and 0.02% pork DNA in binary mixtures.

Conclusions:

  • The gold nanoparticle-based lateral flow assay is a rapid and effective tool for meat authentication.
  • This method offers a practical solution for visual, on-site identification of meat species.
  • The assay's high sensitivity and specificity make it suitable for detecting adulteration in meat products.