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Related Experiment Videos

Initiation of meiosis and sporulation in Saccharomyces cerevisiae does not require a decrease in cyclic AMP.

Z Olempska-Beer, E Freese

    Molecular and Cellular Biology
    |June 1, 1987
    PubMed
    Summary

    Guanine starvation initiates meiosis and sporulation in yeast, challenging the idea that low cyclic adenosine monophosphate (cAMP) is required. Instead, increased cAMP levels and a gluconeogenic carbon source enhance sporulation efficiency.

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    Area of Science:

    • Molecular and Cellular Biology
    • Yeast Genetics and Physiology

    Background:

    • Meiosis and sporulation in Saccharomyces cerevisiae can be induced by guanine deprivation.
    • A previous hypothesis suggested that low cyclic adenosine monophosphate (cAMP) levels are necessary for initiating meiosis.

    Purpose of the Study:

    • To investigate the role of cAMP levels in the initiation of meiosis and sporulation under guanine deprivation.
    • To test the hypothesis that low cAMP is required for meiosis initiation.

    Main Methods:

    • Induction of meiosis and sporulation in guanine auxotroph Saccharomyces cerevisiae via guanine deprivation.
    • Measurement of intracellular cAMP concentrations with and without the cAMP phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX).
    • Assessment of sporulation efficiency and asci formation under various nutrient conditions (guanine, carbon source).

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    Main Results:

    • Guanine deprivation caused only a transient, minor decrease in intracellular cAMP, which was abolished by IBMX.
    • High sporulation rates (≥76%) were achieved with or without IBMX, with nearly 100% sporulation in its presence.
    • Efficient sporulation required a gluconeogenic carbon source; glucose suppressed sporulation without altering cAMP levels.

    Conclusions:

    • cAMP deficiency is not required for initiating meiosis and sporulation under guanine starvation.
    • Saccharomyces cerevisiae produces and excretes excess cAMP during sporulation, and elevated cAMP (via IBMX) improves sporulation.
    • A gluconeogenic carbon source is essential for efficient sporulation, and glucose-mediated suppression is independent of cAMP.