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Updated: Feb 3, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Use of MALDI-TOF MS for Rapid Identification of Clinical Bacterial Strains that Are Uncommon or Difficult to Identify
Naifang Ye1, Boyun Zhang1, Huazhi Ling1
1Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Anhui Medical University, Hefei, Anhui, China.
Abstract:
The purpose of this investigation was to evaluate the analytical and practical performance of MALDI-TOF MS for the identification of clinical uncommon or difficult-to-identify bacteria. A total of 180 clinical isolates collected from January 2014 to December 2016, were identified by MALDI-TOF MS and Vitek2 Compact using 16S rRNA gene sequence analysis as the gold method. For MALDI-TOF MS, 152(84.4%) isolates were correctly identified to the species level, 23(12.8%) isolates were identified to the genus level only, 3(1.7%) were misidentified and 2(1.1%) were unidentified. Compared to those obtained by Vitek2 Compact, 128(71.1%) isolates were correctly identified to the species level, 11(6.1%) isolates were identified to the genus level only, 11(6.1%) were misidentified and 30(16.7%) were unidentified. Vitek2 Compact and MALDI-TOF MS accurately identified 71.1%(128/180), 84.4%(152/180) of the isolates. The time required for the identification by MALDI-TOF MS, Vitek2 Compact, and 16S rRNA gene sequencing was about 10min/strain, 8~12 h/strain and 10 h/ strain. MALDI-TOF MS can be the powerful tools for fast and accurate identification of clinical uncommon or difficult-to-identify bacteria.
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