Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods of Obtaining Topography01:25

Methods of Obtaining Topography

317
Topography involves measuring and mapping land elevations, natural features, and artificial structures to create accurate representations of the terrain. Topographic surveying relies on traditional and modern methods, each with distinct advantages and limitations.Traditional Surveying Methods:Transit stadia surveys and plane table surveys were widely used traditional surveying methods. These techniques relied on instruments like theodolites and stadia rods for measuring distances and angles,...
317
Quantitative Analysis01:12

Quantitative Analysis

1.4K
Quantitative analysis is a technique for measuring the amount of specific constituents in a sample. When the sample's composition is unknown, qualitative analysis is performed first to identify its components, which ensures that the correct substances are measured during the quantitative phase.
In quantitative analysis, two key measurements are made: the sample quantity and a property proportional to the amount of the analyte (the substance being analyzed). This forms the basis of the...
1.4K
Quantitative Aspects of Drug-Receptor Interaction01:30

Quantitative Aspects of Drug-Receptor Interaction

1.8K
The receptor occupancy theory connects a drug's response to the number of occupied receptors. With higher drug concentrations, more receptors are occupied, leading to increased responses. The formation of drug-receptor complexes involves association and dissociation rates, which reach equilibrium when the forward and backward reactions are equal. The equilibrium association constant (Ka) and its inverse, the equilibrium dissociation constant (Kd), indicate drug affinity. Higher Ka and lower...
1.8K
Imaging Studies VII: Vascular Imaging01:19

Imaging Studies VII: Vascular Imaging

371
DefinitionRenal angiography, also known as renal arteriography, is an imaging technique used to obtain a comprehensive view of blood flow and the vascular structure of blood vessels in the kidneys and surrounding areas.PurposeRenal angiography detects blood vessel abnormalities in the kidneys, such as aneurysms, stenosis, thrombosis, vascular tumors, and renal artery stenosis. It evaluates kidney function and guides interventional treatments like angioplasty or stent placement.Pre-Procedure...
371
X-ray Imaging01:24

X-ray Imaging

10.2K
German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with...
10.2K
Brain Imaging01:14

Brain Imaging

743
Brain imaging technologies provide critical insights into both the structure and function of the human brain, enabling medical professionals and researchers to diagnose, study, and treat neurological disorders or psychiatric disorders more effectively.
These technologies include computerized axial tomography (CAT or CT scans), positron-emission tomography (PET scans),  magnetic resonance imaging (MRI),  functional magnetic resonance imaging (fMRI), and Transcranial Magnetic...
743

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SERS Facemask for Rapid and Portable Sensing Mycobacterium Tuberculosis Antigens for TB Screening.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Bilateral SERS-Microneedle Patch for Co-Diagnosis of Diabetes Mellitus and Tuberculosis Comorbidity.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Mycobacterium tuberculosis-induced PCBP1 degradation drives macrophage ferroptosis to promote infection: a lung-macrophage-targeted RNAa nanotherapy.

Journal of nanobiotechnology·2026
Same author

<i>Bacillus subtilis</i> G01: A Multifunctional Agent with Broad-Spectrum Antimicrobial Activity and Digestive Enzymes for Sustainable Agriculture and Animal Husbandry.

Journal of microbiology and biotechnology·2026
Same author

Macrophage targeted cellular microparticles synergize anti-tuberculosis therapy combining CircRNA-mediated host immune defense and antibiotic killing.

Materials today. Bio·2025
Same author

Poly (allylamine hydrochloride)-selenium nanoparticles inhibit porcine reproductive and respiratory syndrome virus by targeting DDX5 and reactive oxygen species.

Antiviral research·2025

Related Experiment Video

Updated: Feb 3, 2026

Quantitative Hardness Measurement by Instrumented AFM-indentation
08:21

Quantitative Hardness Measurement by Instrumented AFM-indentation

Published on: November 22, 2016

10.2K

Cell Topography and Its Quantitative Imaging by AFM.

Jiang Pi1, Jiye Cai2

  • 1State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, China.

Methods in Molecular Biology (Clifton, N.J.)
|October 31, 2018
PubMed
Summary

Atomic Force Microscopy (AFM) offers high-resolution imaging for precise cell topography and quantitative analysis. This work introduces AFM principles, modes, and applications in cell biology, immunology, and medicine.

Keywords:
AFMCellImagingTopography

More Related Videos

Concurrent Quantitative Conductivity and Mechanical Properties Measurements of Organic Photovoltaic Materials using AFM
08:59

Concurrent Quantitative Conductivity and Mechanical Properties Measurements of Organic Photovoltaic Materials using AFM

Published on: January 23, 2013

12.2K
AFM and Microrheology in the Zebrafish Embryo Yolk Cell
09:47

AFM and Microrheology in the Zebrafish Embryo Yolk Cell

Published on: November 29, 2017

8.8K

Related Experiment Videos

Last Updated: Feb 3, 2026

Quantitative Hardness Measurement by Instrumented AFM-indentation
08:21

Quantitative Hardness Measurement by Instrumented AFM-indentation

Published on: November 22, 2016

10.2K
Concurrent Quantitative Conductivity and Mechanical Properties Measurements of Organic Photovoltaic Materials using AFM
08:59

Concurrent Quantitative Conductivity and Mechanical Properties Measurements of Organic Photovoltaic Materials using AFM

Published on: January 23, 2013

12.2K
AFM and Microrheology in the Zebrafish Embryo Yolk Cell
09:47

AFM and Microrheology in the Zebrafish Embryo Yolk Cell

Published on: November 29, 2017

8.8K

Area of Science:

  • Biophysics
  • Cell Biology
  • Nanotechnology

Background:

  • Atomic Force Microscopy (AFM) is a high-resolution imaging technique.
  • AFM provides novel capabilities for cell topography and quantitative imaging.

Purpose of the Study:

  • To introduce the principles and methods of AFM for cell topography and quantitative imaging.
  • To highlight critical tips, applications, and future perspectives of AFM in biological sciences.

Main Methods:

  • Exploration of basic AFM imaging principles.
  • Discussion of fundamental AFM operation modes.
  • Demonstration of AFM imaging on biological samples.

Main Results:

  • Detailed explanation of AFM imaging techniques for biological samples.
  • Identification of critical parameters for successful AFM cell topography.
  • Overview of diverse applications in immunology and medicine.

Conclusions:

  • AFM is a powerful tool for detailed cell surface analysis.
  • Advancements in AFM technology will enhance its utility in biological research.
  • Promotes wider adoption of AFM in cell biology, immunology, and medicine.