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One-Step Photochemical Generation of Biofunctionalized Hydrogel Particles via Two-Phase Flow
Jan-Niklas Schönberg1, Marc Zinggeler1, Patrick Fosso1
1Laboratory for Chemistry and Physics of Interfaces, Department of Microsystems Engineering , University of Freiburg , Georges-Koehler-Allee 103 , Freiburg 79110 , Germany.
Abstract:
Biofunctional hydrogel particles have become increasingly popular in medical diagnostics; however, their generation is time-consuming and typically requires several process steps. We report on a new method for the simple, fast, and reproducible one-step generation of monodisperse hydrogel particles equipped with biofunctional molecules such as proteins or DNA. Key to the approach is the simultaneous photo cross-linking of the polymer chains and covalent binding of proteins or DNA through a C,H insertion reaction inside aqueous plug compartments that are produced via microfluidics. The strong performance in biological binding assays of the functionalized particles is demonstrated.
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