Related Experiment Video

Updated: Feb 3, 2026

Porcine Corneal Tissue Explant to Study the Efficacy of Herpes Simplex Virus-1 Antivirals
08:31

Porcine Corneal Tissue Explant to Study the Efficacy of Herpes Simplex Virus-1 Antivirals

Published on: September 20, 2021

3.8K

Efficacy of Amphotericin B Against Fusarium and Aspergillus in Corneal Storage Medium

Katherine Duncan1, Jeff Parker, Caroline Hoover

  • 1Department of Ophthalmology and Visual Sciences (K.D., B.H.J.), University of Maryland School of Medicine, Baltimore, MD; University of Maryland Pathology Associates, PA (J.P.), Baltimore, MD; and SightLife (C.H., T.D.L.), Seattle, WA.

Eye & Contact Lens
|October 31, 2018
PubMed
Abstract

Insights

Adding amphotericin B to Optisol-GS corneal storage medium significantly reduced fungal growth. This antifungal additive shows promise in preventing postkeratoplasty infections caused by Fusarium solani and Aspergillus fumigatus.

Area of Science:

  • Ophthalmology
  • Mycology
  • Microbiology

Background:

  • Postkeratoplasty fungal infections are an increasing concern.
  • Current corneal storage media like Optisol-GS lack antifungal agents.
  • Previous studies showed amphotericin B effective against Candida albicans.

Purpose of the Study:

  • To evaluate the efficacy of amphotericin B in Optisol-GS against Fusarium solani and Aspergillus fumigatus.
  • To determine if amphotericin B can inhibit common fungal contaminants in corneal storage.

Main Methods:

  • Optisol-GS was supplemented with amphotericin B (0.255 μg/mL).
  • Vials were inoculated with Fusarium solani or Aspergillus fumigatus.
  • Fungal growth was assessed by colony counts after incubation.

Main Results:

  • Amphotericin B reduced Fusarium solani growth by 44% (day 2) to 80% (day 14).
  • Amphotericin B reduced Aspergillus fumigatus growth by 40% (day 2), with minimal growth by day 14.
  • Both supplemented and control vials showed limited Aspergillus fumigatus growth by day 14.

Conclusions:

  • Amphotericin B additives in Optisol-GS effectively reduce the growth of Fusarium solani.
  • Amphotericin B demonstrates inhibitory effects against Aspergillus fumigatus in corneal storage medium.

More Related Videos

Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung07:46

Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung

Published on: June 5, 2012

34.1K
A Porcine Corneal Endothelial Organ Culture Model Using Split Corneal Buttons08:36

A Porcine Corneal Endothelial Organ Culture Model Using Split Corneal Buttons

Published on: October 6, 2019

7.6K

Related Concept Videos

Storage01:23

Storage

A schema is a mental framework that helps individuals organize and interpret information. Schemata, formed from previous experiences, influence how we process new information: how we encode it, the inferences we make, and how we retrieve it. For instance, a schema for what a typical classroom looks like might include desks, a teacher's desk, a whiteboard, and students in such an environment. This expectation helps us quickly understand and navigate new classrooms without needing to analyze...
410
Self-Efficacy01:29

Self-Efficacy

Self-efficacy is the belief in one's capacity to organize and execute actions necessary to manage prospective situations. This belief significantly influences how individuals approach goals, tasks, and challenges across different domains of life.Psychological and Educational ImpactsIndividuals with strong self-efficacy are more resilient in the face of difficulties. They are more likely to adopt effective problem-solving strategies, persist through obstacles, and regulate emotions such as...
227
ATP Energy Storage and Release01:31

ATP Energy Storage and Release

ATP is a highly unstable molecule. Unless quickly used to perform work, ATP spontaneously dissociates into ADP and inorganic phosphate (Pi), and the free energy released during this process is lost as heat. The energy released by ATP hydrolysis is used to perform work inside the cell and depends on a strategy called energy coupling. Cells couple the exergonic reaction of ATP hydrolysis with endergonic reactions, allowing them to proceed.
One example of energy coupling using ATP involves a...
14.4K
Sugars as Energy Storage Molecules01:10

Sugars as Energy Storage Molecules

Sugar (a simple carbohydrate) metabolism (chemical reactions) is a classic example of the many cellular processes that use and produce energy. Living things consume sugar as a major energy source because sugar molecules have considerable energy stored within their bonds. Consumed carbohydrates have their origins in photosynthesizing organisms like plants. During photosynthesis, plants use the energy of sunlight to convert carbon dioxide gas into sugar molecules, like glucose. Because this...
9.9K
Fats as Energy Storage Molecules01:06

Fats as Energy Storage Molecules

Triglycerides are a form of long-term energy storage molecules. They are made of glycerol and three fatty acids. To obtain energy from fat, triglycerides must first be broken down by hydrolysis into their two principal components, fatty acids and glycerol. This process, called lipolysis, takes place in the cytoplasm. The resulting fatty acids are oxidized by β-oxidation into acetyl-CoA, which is used by the Krebs cycle. The glycerol that is released from triglycerides after lipolysis...
27.0K
Dose-Response Relationship: Potency and Efficacy01:22

Dose-Response Relationship: Potency and Efficacy

The potency of a drug is the measure of its ability to produce a biological response and can be compared by looking at the half-maximum effective concentration or EC50 values of different drugs. A lower EC50 value indicates higher potency of the drug. In the dose–response curve of two antihypertensive drugs, candesartan and irbesartan, a significant difference is observed in their EC50 values. A lower EC50 value for candesartan indicates that it is more potent than irbesartan, as it...
6.5K