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Updated: Feb 3, 2026

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
Published on: May 16, 2017
A fully automatic method yielding initial models from high-resolution cryo-electron microscopy maps
Thomas C Terwilliger1,2, Paul D Adams3,4, Pavel V Afonine3,5
1Los Alamos National Laboratory, Los Alamos, NM, USA. tterwilliger@newmexicoconsortium.org.
Abstract:
We report a fully automated procedure for the optimization and interpretation of reconstructions from cryo-electron microscopy (cryo-EM) data, available in Phenix as phenix.map_to_model. We applied our approach to 476 datasets with resolution of 4.5 Å or better, including reconstructions of 47 ribosomes and 32 other protein-RNA complexes. The median fraction of residues in the deposited structures reproduced automatically was 71% for reconstructions determined at resolutions of 3 Å or better and 47% for those at resolutions worse than 3 Å.
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