FLIRT: fast local infrared thermogenetics for subcellular control of protein function
Sophia M Hirsch1, Sriramkumar Sundaramoorthy2,3, Tim Davies2
1Department of Genetics and Development, Columbia University Medical Center, New York, NY, USA.
Abstract:
FLIRT (fast local infrared thermogenetics) is a microscopy-based technology to locally and reversibly manipulate protein function while simultaneously monitoring the effects in vivo. FLIRT locally inactivates fast-acting temperature-sensitive mutant proteins. We demonstrate that FLIRT can control temperature-sensitive proteins required for cell division, Delta-Notch cell fate signaling, and germline structure in Caenorhabditis elegans with cell-specific and even subcellular precision.
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