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Spatial organization of the somatosensory cortex revealed by osmFISH
Simone Codeluppi1,2,3, Lars E Borm4,5, Amit Zeisel4,5,6
1Division of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden. simone.codeluppi@ki.se.
Abstract:
Global efforts to create a molecular census of the brain using single-cell transcriptomics are producing a large catalog of molecularly defined cell types. However, spatial information is lacking and new methods are needed to map a large number of cell type-specific markers simultaneously on large tissue areas. Here, we describe a cyclic single-molecule fluorescence in situ hybridization methodology and define the cellular organization of the somatosensory cortex.
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