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Updated: Feb 3, 2026

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
Published on: January 17, 2015
A high-throughput pipeline for validation of antibodies
Krzysztof Sikorski1,2, Adi Mehta1,3,4, Marit Inngjerdingen1
1Department of Immunology, University of Oslo and Oslo University Hospital, Oslo, Norway.
Abstract:
Western blotting (WB) is widely used to test antibody specificity, but the assay has low throughput and precision. Here we used preparative gel electrophoresis to develop a capture format for WB. Fractions with soluble, size-separated proteins facilitated parallel readout with antibody arrays, shotgun mass spectrometry (MS) and immunoprecipitation followed by MS (IP-MS). This pipeline provided the means for large-scale implementation of antibody validation concepts proposed by an international working group on antibody validation (IWGAV).
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