Related Experiment Video
Updated: Feb 16, 2026

Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone
Published on: June 24, 2019
Molecular cloning of infectious proviral genomes of bovine leukemia virus
Abstract:
Covalently closed circular DNA molecules of bovine leukemia virus were cloned in the lambda phage vector lambda gtWES-lambda B and subsequently in the plasmid vector pUC12. Proviral DNAs of 8.3 kb which have one copy of a long terminal repeat and uniform restriction endonuclease sites were preferentially obtained. Four randomly selected clones were examined for their biological activities by DNA transfection experiments. The ovine embryonic cells transfected with these clones formed syncytia which represent the expression of BLV genes. The infectious virions could be recovered from the transfectants.
More Related Videos
Related Concept Videos
Viruses with RNA Genomes
Reproductive Cloning
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic...
What are Viruses?
Genomics
Retroviruses
Cloning of Dolly the Sheep

