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Updated: Feb 3, 2026

Analysis of Oxidative Stress in Zebrafish Embryos
Published on: July 7, 2014
Cell Death or Survival Against Oxidative Stress
Non Miyata1,2, Kanji Okumoto1,3, Yukio Fujiki4
1Department of Biology, Faculty of Sciences, Kyushu University, 819-0395, Nishi-ku, Fukuoka, Japan.
Abstract:
Peroxisomes contain anabolic and catabolic enzymes including oxidases that produce hydrogen peroxide as a by-product. Peroxisomes also contain catalase to metabolize hydrogen peroxide. It has been recognized that catalase is localized to cytosol in addition to peroxisomes. A recent study has revealed that loss of VDAC2 shifts localization of BAK, a pro-apoptotic member of Bcl-2 family, from mitochondria to peroxisomes and cytosol, thereby leading to release of peroxisomal matrix proteins including catalase to the cytosol. A subset of BAK is localized to peroxisomes even in wild-type cells, regulating peroxisomal membrane permeability and catalase localization. The cytosolic catalase potentially acts as an antioxidant to eliminate extra-peroxisomal hydrogen peroxide.
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