Pancreatic Microtumors: A Novel 3D Ex Vivo Testing Platform
Mackenzie Lee Goodwin1, Sumi Kanthraj Urs1, Diane M Simeone2,3
1Department of Surgery, University of Michigan, Ann Arbor, MI, USA.
Abstract:
The tumor microenvironment plays a vital role in therapeutic resistance in pancreatic adenocarcinoma. We have created a 3D model to study the tumor microenvironment ex vivo that integrates tumor cells with tumor associated macrophages (TAMs) and cancer associated fibroblasts (CAFs). The microtumor model recreates the complex histology of a primary tumor and can be analyzed histologically or with viability assays for therapeutic testing. Microtumors demonstrate increased resistance to gemcitabine when compared to tumor cells alone in a 3D spheroid, highlighting the contribution of TAMs and CAFs when designing models for therapeutic testing.
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