Related Experiment Video
Updated: Feb 3, 2026

Ultrasound-Guided Orthotopic Implantation of Murine Pancreatic Ductal Adenocarcinoma
Published on: November 19, 2019
3D Models of Pancreatic Ductal Adenocarcinoma via Tissue Engineering
1Department of Civil and Industrial Engineering, University of Pisa, Pisa, Italy.
Abstract:
Cancer tissue engineering is an emerging multidisciplinary field aimed at growing cancerous cells onto porous biomaterial scaffolds and proper stimuli to ultimately reproduce 3D tumor tissue-like constructs in vitro. Unlike conventional 2D cell cultures and spheroids, these tissue models can reproduce cancer lesions very similar to those present in native tumor, and can be viable for some weeks, making it possible to study cancer biology phenomena and new therapies in a more reliable fashion than with conventional in vitro platforms. This chapter shows the preparation of a 3D model of pancreatic ductal adenocarcinoma (PDAC), including fabrication of a suitable scaffold, culture of PDAC cells on the scaffold, viability test, and histologic assessment.
More Related Videos
Related Concept Videos
What is Genetic Engineering?
Heat Engines
Whenever we consider heat engines (and associated devices such as refrigerators and heat pumps), we do not use the standard sign convention for heat and work. For convenience, we assume that the symbols Qh, Qc, and W represent only the amounts of heat transferred...
Pancreatic Juice and Secretion
When acidic chyme from the stomach enters the duodenum, it triggers the release of secretin, a hormone that prompts pancreatic juice secretion. After a fatty meal, cholecystokinin, another hormone, stimulates gallbladder contraction and enhances enzyme-rich...
Acute Pancreatitis I: Introduction
Acute pancreatitis is characterized by rapid inflammation of the pancreas, often caused by factors like gallstone blockage or excessive alcohol consumption. Chronic pancreatitis, on the other hand, is a slow, progressive inflammation that may result from long-term alcohol abuse, obstructions in the pancreatic duct, or genetic factors.
The causes of acute pancreatitis include:
Chronic Pancreatitis I: Introduction
Pancreatitis is the inflammation of the pancreas, which occurs when the immune system becomes active and causes swelling, pain, and disruptions in organ function. Pancreatitis can manifest as either an acute or chronic condition.
Acute pancreatitis arises suddenly and lasts for a brief duration, while chronic pancreatitis is a long-term affliction...
Internal Combustion Engine

