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Published on: April 11, 2012
[The Establishment of Tunica Media-induced Systemic Inflammatory Responses Animal Model]
Jun Gu1, Wei Meng1, Chang-Ping Gan1
1Department of Cardiovascular Surgery, West China Hospital, Sichaun University, Chengdu 610041, China.
Objective:
To establish animal model of tunica media-induced systemic inflammatory responses and verify the stability of established model.
Methods:
Tunica media of aorta was captured from C57/BL6 mouse donor, followed by the intervention of homogenization. After centrifugation, the supernatant was harvested and injected into the acceptor of C57/BL6 mouse to mimic the featured pathological changes induced by the exposure of tunica media with circulation. ELISA assay was used to detect the levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).
Results:
The levels of TNF-α, IL-6 in the model group were significant higher than those in the control group (P<0.05). There were two peak values of both TNF-α and IL-6 identified at 3 h and 24 h after the injection of supernatant in the model group.
Conclusion:
The exposure of tunica media to circulation may induce systemic inflammatory responses.
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