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Updated: Feb 3, 2026
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Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
Published on: October 5, 2011
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Systems-Wide Approaches in Induced Pluripotent Stem Cell Models
Edward Lau1, David T Paik1, Joseph C Wu1,2
1Stanford Cardiovascular Institute, and Department of Medicine, Division of Cardiology, Stanford University, Stanford, California 94305, USA;
Annual Review of Pathology
|November 1, 2018
Summary
Human induced pluripotent stem cells (iPSCs) offer a valuable resource for studying diseases. Advances in omics technologies and iPSC models enable comprehensive molecular profiling for systems biology approaches.
Area of Science:
- Biotechnology
- Genomics
- Systems Biology
Background:
- Human induced pluripotent stem cells (iPSCs) provide a renewable source of patient-specific cells.
- iPSCs are crucial for studying disease mechanisms at cellular and molecular levels.
- Recent advancements have generated large panels of iPSC lines from diverse individuals.
Purpose of the Study:
- To review recent developments in omics profiling of molecular phenotypes using iPSCs.
- To highlight the role of human iPSCs as a systems biology model for human diseases.
Main Methods:
- Utilizing omics technologies (genomics, transcriptomics, proteomics, metabolomics) for molecular profiling.
- Deriving and characterizing large panels of human iPSC lines from genetically heterogeneous individuals.
- Genome-wide mapping to identify coexpression and gene regulatory networks.
Main Results:
- Omics profiling of iPSCs allows detailed characterization of molecular phenotypes.
- Large iPSC panels facilitate genome-wide analyses for network identification.
- Human iPSCs serve as a powerful model for systems biology studies of diseases.
Conclusions:
- Human iPSCs combined with omics technologies are instrumental in advancing our understanding of disease mechanisms.
- The development of iPSC models represents a significant step forward in systems biology approaches to human diseases.
- Future research can leverage iPSC-based systems biology for personalized medicine and therapeutic development.
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