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Updated: Feb 3, 2026
07:11
Homemade Site Directed Mutagenesis of Whole Plasmids
Published on: May 11, 2009
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PLSDB: a resource of complete bacterial plasmids
Valentina Galata1, Tobias Fehlmann1, Christina Backes1
1Chair for Clinical Bioinformatics, Saarland University, Campus Building E2.1, 66123 Saarbruecken, Germany.
Nucleic Acids Research
|November 1, 2018
Summary
PLSDB is a new online resource for analyzing bacterial plasmids, including those with antibiotic resistance genes. This database helps researchers track plasmid diversity and evolution in microbial communities.
Area of Science:
- Microbiology
- Bioinformatics
- Genomics
Background:
- Plasmid analysis is crucial for comprehensive characterization of bacterial isolates and communities.
- Plasmids carrying resistance and virulence factors are key drivers of antibiotic resistance dissemination.
- The increasing volume of sequenced bacterial genomes necessitates a centralized resource for plasmid data management and analysis.
Purpose of the Study:
- To introduce PLSDB, a comprehensive database of bacterial plasmids.
- To provide a web server for browsing, filtering, and analyzing plasmid sequences.
- To facilitate the identification and characterization of plasmids within bacterial samples.
Main Methods:
- Compiled 13,789 plasmid records from the NCBI nucleotide database.
- Developed a web server with interactive plasmid visualization and meta-information.
- Implemented sequence analysis tools for searching genes, comparing plasmids, and performing containment analysis.
Main Results:
- PLSDB offers an extensive collection of bacterial plasmids with associated metadata.
- The platform enables interactive exploration of plasmid sequence characteristics, sample origins, and taxonomy.
- Users can upload sequences for targeted searches, comparative analysis, and containment assessments.
Conclusions:
- PLSDB serves as a valuable, freely accessible resource for the microbiology and bioinformatics communities.
- The database and associated tools streamline the analysis of plasmids, aiding in the study of bacterial genetics and evolution.
- PLSDB supports research on antibiotic resistance by facilitating the tracking of relevant plasmid-borne factors.
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