Deactivation of E. coli in water using Fe³⁺-saturated montmorillonite impregnated filter paper

In areas with high exposure to pathogen contaminated water and lack the economic means for water treatment, low cost and convenient point-of-use drinking water disinfection materials/devices are essential. Using a simple craft paper making method, Fe³⁺-saturated montmorillonite impregnated filter paper was constructed to filter live Escherichia coli (E. coli)-spiked water. The Scanning Electron Microscopic images of the E. coli cells in contact with the Fe³⁺-saturated montmorillonite impregnated filter paper showed: 1) Fe³⁺-saturated montmorillonite particles were uniformly coated on the cellulose paper fiber, creating large mineral surface for cell contact; and 2) E. coli cell membrane was dehydrated and damaged, resulting cell deactivation upon contacting with the Fe³⁺-saturated montmorillonite particles impregnated in the paper. The E. coli cells passing through the Fe³⁺-saturated montmorillonite impregnated filter paper were not viable as further confirmed by the microfluidic dielectrophoresis analysis. They remained non-viable at room temperature even after 5 days, as shown by the results from both the Colony Counting test and the Colilert test. More than 99.5% deactivation efficiency was achieved when the ratio of the volume of the E. coli contaminated water to the mass of Fe³⁺-saturated montmorillonite was maintained at <1:1.5 (mL/mg). The Fe³⁺-saturated montmorillonite impregnated filter paper maintained ~74% E. coli deactivation efficiency even after the 8th consecutive use. About 0.52 mg Fe³⁺, which is bioavailable, could be leached into the water for every 2 L E coli-contaminated water that is treated with the filter paper. The treated water could therefore provide iron supplement to a person at a level within the range of the FDA recommended human daily intake of iron. The results from this study has clearly demonstrated promising potential of using the Fe³⁺-saturated montmorillonite impregnated filter paper for low cost (~$0.07/L treated water for this study) and convenient point-of-use drinking water disinfection.