Oestradiol binding activity in rat liver tumours and in human hepatocellular carcinoma

Oestradiol binding activity was studied in normal liver tissues of rats as well as in experimentally induced liver tumours by incubating a cytosol prepared from the tissue with radioactive oestradiol. The free radioactive hormone was absorbed by a dextran-charcoal suspension, and the amount required to saturate the receptors was calculated by a Scatchard plot. Similar activity was observed in normal human liver as well as naturally occurring hepatocellular carcinoma.