Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hepatocarcinogens induce decrease in mRNA transcripts of receptors for insulin and epidermal growth factor in the rat

D L Hwang, A Lev-Ran, Y C Tay

    Biochemical and Biophysical Research Communications
    |July 15, 1987
    PubMed
    Summary

    Hepatocarcinogens like diethylnitrosamine (DEN) and N-acetylaminofluorene (AAF) significantly decrease gene expression for insulin and epidermal growth factor (EGF) receptors in rat livers. This reduction occurs early and persists, even in tumors, suggesting a key role in liver cancer development.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Beyond the Coral Triangle: high genetic diversity and near panmixia in Singapore's populations of the broadcast spawning sea star <i>Protoreaster nodosus</i>.

    Royal Society open science·2016
    Same author

    Leiomyoma of the oesophagus managed by thoracoscopic enucleation.

    Singapore medical journal·2006
    Same author

    Divide-and-conquer approach for the exemplar breakpoint distance.

    Bioinformatics (Oxford, England)·2005
    Same author

    Early administration of PDTC in adriamycin nephropathy: effect on proteinuria, cortical tubulointerstitial injury, and NF-kappaB activation.

    Renal failure·2002
    Same author

    Human obesity: an evolutionary approach to understanding our bulging waistline.

    Diabetes/metabolism research and reviews·2001
    Same author

    Role of CD8(+) cells in the progression of murine adriamycin nephropathy.

    Kidney international·2001

    Area of Science:

    • Hepatology
    • Molecular Biology
    • Toxicology

    Background:

    • Insulin and epidermal growth factor (EGF) receptors play crucial roles in liver function and cell growth.
    • Hepatocarcinogens are known to disrupt normal liver processes, potentially affecting receptor expression.

    Purpose of the Study:

    • To investigate the impact of hepatocarcinogens diethylnitrosamine (DEN) and N-acetylaminofluorene (AAF) on the gene expression of insulin and EGF receptors in rat livers.
    • To understand the early and late effects of these carcinogens on receptor mRNA levels.

    Main Methods:

    • Rats were administered DEN or fed AAF, known hepatocarcinogens.
    • Liver samples were analyzed for the mRNA transcription levels of insulin and EGF receptors at various time points.
    • Hepatocellular carcinomas were monitored in DEN-treated rats one year post-injection.

    Related Experiment Videos

    Main Results:

    • Both DEN and AAF induced a rapid and significant decrease in mRNA transcription for both insulin and EGF receptors.
    • DEN caused a time-dependent decrease, reaching a nadir within 16-24 hours, with levels returning to normal by 10 days.
    • In rats that developed hepatocellular carcinomas, expression of both receptors remained very low, while DNA showed no changes.

    Conclusions:

    • Hepatocarcinogens or their metabolites likely decrease RNA transcription or destabilize RNA levels of insulin and EGF receptors during the early toxic phase.
    • Tumor-derived products may contribute to the sustained low expression of these receptors in later stages of liver tumor development.