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Updated: Feb 3, 2026

Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae
Published on: October 8, 2013
Rapid Detection of Fosfomycin Resistance in Escherichia coli
Patrice Nordmann1,2,3,4, Laurent Poirel5,2,3, Linda Mueller5,2
1Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland patrice.nordmann@unifr.ch.
Abstract:
The rapid fosfomycin/Escherichia coli NP test was developed to detect fosfomycin resistance in E. coli isolates. The test is based on glucose metabolization and the detection of bacterial growth in the presence of fosfomycin at 40 µg/ml. Bacterial growth is visually detectable by an orange-to-yellow color change of red phenol, a pH indicator. A total of 100 E. coli isolates, among which 22 were fosfomycin resistant, were used to evaluate the test performance. The sensitivity and specificity of the test were 100% and 98.7%, respectively. This new test is user friendly, sensitive and specific, and its results are obtained in 1 h 30 min.
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