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Antifungal activity of magnoflorine against Candida strains

Jaegoo Kim1, Thinh Ha Quang Bao1, Yu-Kyong Shin2

  • 1Graduate School of Biotechnology, Kyung Hee University, Yongin-si, Gyeonggi-do, Republic of Korea.

World Journal of Microbiology & Biotechnology
|November 2, 2018
PubMed

Insights

Magnoflorine effectively inhibits Candida albicans growth and biofilm formation, showing potential as a novel antifungal agent. It demonstrates low toxicity and enhances conventional antifungal drug efficacy.

Area of Science:

  • Microbiology
  • Pharmacology
  • Natural Products Chemistry

Background:

  • Candida albicans is a significant invasive fungal pathogen.
  • Antifungal resistance in Candida strains is a growing clinical concern.
  • There is a need for novel antifungal agents with improved efficacy and safety profiles.

Purpose of the Study:

  • To evaluate the antifungal activity of 44 compounds against Candida strains.
  • To identify potential lead compounds for new antifungal drug development.
  • To investigate the mechanism of action and safety of promising compounds.

Main Methods:

  • Microdilution antifungal susceptibility testing to determine minimum inhibitory concentration (MIC).
  • Disk diffusion assays to assess antifungal activity and stability.
  • Cytotoxicity testing on HaCaT cells to evaluate safety.
  • Enzyme inhibition assays to determine alpha-glucosidase activity.
  • Biofilm formation assays.
  • Combination treatment studies with miconazole.

Main Results:

  • Magnoflorine exhibited the highest growth inhibitory activity against Candida strains, with an MIC of 50 μg/mL.
  • Antifungal activity of magnoflorine was stable over 3 days.
  • Magnoflorine showed no toxicity to HaCaT cells at concentrations up to 200 μg/mL.
  • Magnoflorine inhibited 55.91 ± 7.17% of alpha-glucosidase activity at 50 μg/mL.
  • Magnoflorine reduced Candida albicans biofilm formation.
  • Combined treatment with magnoflorine and miconazole enhanced the efficacy of miconazole.

Conclusions:

  • Magnoflorine is a potent inhibitor of Candida albicans growth and virulence factors.
  • Magnoflorine possesses a favorable safety profile for potential clinical applications.
  • Magnoflorine demonstrates synergistic effects when combined with conventional antifungals.
  • Magnoflorine represents a promising candidate lead compound for the development of novel antifungal therapies.
Keywords:
Alpha-glucosidaseAntifungalCandida albicansMagnoflorineSusceptibility microdilution assay

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