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Proteomics in Systems Biology.

Wan Mohd Aizat1, Maizom Hassan2

  • 1Institute of Systems Biology, Universiti Kebangsaan Malaysia (UKM), Bangi, Malaysia. wma@ukm.edu.my.

Advances in Experimental Medicine and Biology
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Summary

This chapter explores expression proteomics, a key area of protein study. It details the workflow, mass spectrometry methods, and applications in cancer and crop research.

Keywords:
EnzymeExpression proteomicsMass spectrometryPeptideProteinShotgun proteomics

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Area of Science:

  • Proteomics: The comprehensive study of proteins and their dynamic post-translational modifications.

Background:

  • Proteins are fundamental cellular components, exhibiting dynamic changes in response to stimuli.
  • Proteomics encompasses sequence, structural, functional, interaction, and expression analyses, each with distinct methodologies and objectives.

Purpose of the Study:

  • To detail the applications of expression proteomics in cancer and crop research.
  • To describe the general workflow of expression proteomics, including mass spectrometry techniques.

Main Methods:

  • Utilizing mass spectrometry for protein identification and quantification.
  • Employing various proteomic approaches: sequence, structural, functional, interaction, and expression.

Main Results:

  • Expression proteomics enables the comprehensive cataloguing of proteins within biological samples.
  • Demonstrates the utility of proteomics in advancing cancer and crop research through holistic protein overviews.

Conclusions:

  • Expression proteomics provides a global view of cellular proteins, crucial for understanding complex biological systems.
  • The described workflow and applications highlight the power of proteomics in diverse research fields.