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Updated: Feb 3, 2026

Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR
Published on: July 8, 2025
NMR supersequences with real-time homonuclear broadband decoupling: Sequential acquisition of protein and small
Veera Mohana Rao Kakita1, Kavitha Rachineni1, Mandar Bopardikar2
1UM-DAE Centre for Excellence in Basic Sciences, University of Mumbai, Kalina Campus, Santacruz, Mumbai 400 098, India.
Abstract:
NOAH (NMR byOrderedAcquisition using 1H-detection) type of pure shift NMR pulse scheme has been designed for the efficient utilization of magnetization that presents in a spin-system under consideration. The proposed strategy, PROSMASH-HSQC2 (PROtein-HSQC and SMAll molecule-HSQC Signals with Homodecoupling) uses the real-time BIRD pure shift NMR strategy and two HSQC spectra (13C-HSQC for small molecules and 15N-HSQC for 15N-isotopic labelled proteins) can be recorded in a single NMR experiment. Thus, this method permits precise determination of drug-protein interactions at atomic levels by monitoring the chemical shift perturbations, and will have potential applications in drug discovery programs.
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