Related Experiment Video
Updated: Feb 3, 2026

Bio-inspired Polydopamine Surface Modification of Nanodiamonds and Its Reduction of Silver Nanoparticles
Published on: November 14, 2018
Stabilization of polydopamine modified silver nanoparticles bound trypsin: Insights on protein hydrolysis
Irfanah Siddiqui1, Qayyum Husain1
1Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, 202002, India.
Abstract:
This study presents an effective method to enhance the proteolytic activity of trypsin by designing a nanobioconjugate of trypsin and polydopamine coated silver nanoparticles (Ag-PDA). Ag-PDA nanocomposite was synthesized and trypsin was covalently attached to this matrix. A multifold enhancement in enzyme activity was observed after conjugation, with an effectiveness factor of η = 5.62. Conjugated trypsin was more stable to extremes of pH and temperature as compared to normal unconjugated version. Unconjugated trypsin retained only 41% of activity after 60 days of storage at 4 °C, whereas the conjugated enzyme preserved 91% of activity. Immobilized trypsin conserved 83% of activity even after its ten repeated uses. It was found that conjugated trypsin required lower incubation periods for complete hydrolysis of casein, BSA & ovalbumin as compared to free enzyme. Contrary to this, even long hours of incubation with free trypsin were unable to completely digest these proteins. The current observations suggest that Ag-PDA conjugated trypsin could be more suitable for efficient hydrolysis of various proteins under industrial environments.
Related Concept Videos
Hydrolysis of ATP
If one phosphate group is removed, a molecule of ADP—adenosine diphosphate—remains, along with inorganic phosphate. ADP can be further hydrolyzed to AMP—adenosine...
Hydrolysis
Hydrolysis is a chemical reaction in which the addition of water breaks down a polymer into its simpler monomer units. For example, peptides break into amino acids, carbohydrates into simple sugars, and DNA into nucleotides. Enzymes often facilitate these processes.
Hydrolysis Reverses Dehydration Synthesis
Complex carbohydrates can be broken down by breaking the bonds between individual sugar units. The reaction breaks a glycosidic bond as water is added to the compound. The...
RNA Stability
Mechanical Protein Functions
Nuclear Stability
To hold positively charged protons together...
Molecular Chaperones and Protein Folding
The...

