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Alcohol hangover effects on brain cortex non-synaptic mitochondria and synaptosomes bioenergetics
Analía G Karadayian1, Paulina Lombardi1, Juanita Bustamante2
1Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Fisicoquímica, Buenos Aires, Argentina; CONICET-Universidad de Buenos Aires, Instituto de Bioquímica y Medicina Molecular (IBIMOL), Buenos Aires, Argentina.
Alcohol (Fayetteville, N.Y.)
|November 3, 2018
Summary
Alcohol hangover impairs brain mitochondrial function, particularly at synapses. This study reveals distinct effects on synaptosomes versus non-synaptic mitochondria, impacting energy production and cellular respiration during hangover.
Area of Science:
- Neuroscience
- Mitochondrial Biology
- Toxicology
Background:
- Alcohol hangover (AH) is linked to oxidative stress and mitochondrial dysfunction.
- Distinct responses in synaptosomes and non-synaptic (NS) mitochondria during AH are hypothesized.
Purpose of the Study:
- To investigate the differential effects of alcohol hangover on the bioenergetics of synaptosomes and NS mitochondria in the brain.
- To elucidate the specific mitochondrial alterations contributing to hangover symptoms.
Main Methods:
- Mice were administered ethanol (3.8 g/kg) or saline, with brain cortex NS mitochondria and synaptosomes isolated 6 hours post-injection.
- High-resolution respirometry was used to measure oxygen consumption rates, ATP content, and mitochondrial complex activities.
Main Results:
- NS-mitochondria showed decreased respiration and ATP production, with inhibited complex IV activity.
- Synaptosomes exhibited altered respiration patterns, reduced ATP content, and decreased activities of mitochondrial respiratory complexes I-III, II-III, and IV.
- Synaptosomes displayed a decrease in mitochondrial transmembrane potential (ΔΨ) and an increase in UCP-2 expression.
Conclusions:
- Alcohol hangover significantly impacts brain mitochondrial function, with more pronounced detrimental effects observed at the synaptic level.
- Alterations in synaptic mitochondrial bioenergetics are a primary contributor to the physiological state of alcohol hangover.
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