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Related Experiment Videos

Directed Ig class switch recombination in activated murine B cells.

E Winter, U Krawinkel, A Radbruch

    The EMBO Journal
    |June 1, 1987
    PubMed
    Summary

    Immunoglobulin class switch recombination in B-cells involves gene rearrangements and deletions. Interleukin 4 may direct specific switch recombination events in murine B-lymphocytes.

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    Immunomodulation by vitamin D.

    Allergologie select·2019

    Area of Science:

    • Immunology
    • Molecular Biology
    • Genetics

    Background:

    • Immunoglobulin class switch recombination (CSR) is a crucial process for adaptive immunity, occurring at high frequencies in activated murine B-lymphocytes.
    • CSR enables B-cells to switch from producing IgM to other immunoglobulin isotypes (IgG, IgA, IgE), enhancing antibody effector functions.

    Purpose of the Study:

    • To analyze immunoglobulin heavy chain (IgH) gene rearrangements and switch recombination events in activated murine B-cells.
    • To investigate the fate of the Cμ gene during CSR and its association with specific switch regions.
    • To explore the potential role of Interleukin 4 (IL-4) in directing CSR.

    Main Methods:

    • Analysis of Cμ gene rearrangements and switch recombination in activated and immortalized murine B-cells.
    • Examination of both active and inactive IgH loci to assess gene segment deletions and rearrangements.
    • Fusion of B-cells to create immortalized cell lines for detailed molecular analysis.

    Main Results:

    • Approximately half of IgM+ cells showed Cμ gene rearrangement, while Cμ deletion was infrequent.
    • In IgG3+ and IgG1+ cells, half exhibited Cμ gene rearrangement on the inactive IgH locus.
    • The other half of IgG+ cells had deleted Cμ from both IgH loci via switch recombination, with 60-80% showing recombination at identical switch regions on both loci.

    Conclusions:

    • Switch recombination leads to Cμ gene deletion in a significant proportion of IgG+ B-cells.
    • The observed coordinated recombination at identical switch regions on both IgH loci suggests a regulated process.
    • Interleukin 4 may play a critical role in programming B-lymphocytes for specific switch recombination pathways.

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