Embryonic Immunostaining for the Tardigrade Hypsibius exemplaris
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Updated: Feb 3, 2026

Single-Animal, Single-Tube RNA Extraction for Comparison of Relative Transcript Levels via qRT-PCR in the Tardigrade Hypsibius exemplaris
Published on: January 3, 2025
Frank W Smith1, Willow N Gabriel2
1Biology Department, University of North Florida, Jacksonville, Florida 32224; frank.smith@unf.edu.
Abstract:
Immunostaining is a method used to visualize the localization of proteins in fixed tissue. Many antibodies are available that recognize specific proteins in a wide diversity of organisms, which makes this method ideal for investigating gene expression patterns in nonmodel animal systems. This protocol describes immunostaining for studies of embryogenesis in the tardigrade Hypsibius exemplaris.
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