Related Experiment Video

Updated: Feb 3, 2026

Orthotopic Transplantation of Syngeneic Lung Adenocarcinoma Cells to Study PD-L1 Expression
06:03

Orthotopic Transplantation of Syngeneic Lung Adenocarcinoma Cells to Study PD-L1 Expression

Published on: January 19, 2019

18.2K

Thymoquinone-induced antitumor and apoptosis in human lung adenocarcinoma cells

Saeed Samarghandian1, Mohsen Azimi-Nezhad1,1, Tahereh Farkhondeh2

  • 1Department of Basic Medical Science, Neyshabur University of Medical Sciences, Neyshabur, Iran.

Journal of Cellular Physiology
|November 3, 2018
PubMed
Abstract

Insights

Thymoquinone (TQ), derived from black seed, shows potential as a lung cancer therapeutic. It effectively reduced cancer cell viability and induced apoptosis through p53 and caspase pathways.

Area of Science:

  • Oncology
  • Pharmacology
  • Natural Products

Background:

  • Lung cancer is a leading cause of cancer mortality globally.
  • Exploring novel chemotherapeutic agents from natural sources is crucial.
  • Thymoquinone (TQ), from Nigella sativa, exhibits antioxidant and anti-inflammatory properties.

Purpose of the Study:

  • To investigate the antineoplastic effects of TQ on human lung cancer cells (A549).
  • To elucidate the mechanisms underlying TQ's anti-cancer activity.

Main Methods:

  • A549 cells were treated with varying concentrations of TQ.
  • Cell viability was assessed using MTT assay.
  • Apoptosis, necrosis, caspase activation (caspase-3, -9), and DNA fragmentation were analyzed.

Main Results:

  • TQ significantly reduced A549 cell viability.
  • TQ induced apoptosis, evidenced by increased Bax/Bcl-2 ratio and p53 upregulation.
  • TQ activated caspase-dependent apoptosis pathways.

Conclusions:

  • TQ demonstrates potential as a novel therapeutic agent for lung cancer.
  • TQ promotes apoptosis in lung cancer cells via p53 and caspase activation.
Keywords:
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)A549BaxBcl-2antiproliferativecaspasesp53thymoquinone (TQ)

More Related Videos

Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma13:18

Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma

Published on: March 3, 2023

1.8K
Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen19:44

Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen

Published on: May 30, 2012

19.2K

Related Concept Videos

Apoptosis01:30

Apoptosis

Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
14.5K
Lung Capacity01:47

Lung Capacity

The air in the lungs is measured in volumes and capacities. Lung volume measures reflect the amount of air taken in, released, or left over after a lung function, like a single inhalation. Lung capacity measures are sums of two or more lung volume measures.
56.3K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.0K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
5.6K
Pleura of the Lungs01:13

Pleura of the Lungs

The lungs are nestled in a cavity, shielded by the pleura. The pleura, a form of serous membrane, wraps around each lung. This membrane arrangement consists of two layers: the visceral and parietal pleurae. The visceral pleura lines the surface of the lungIn contrast, the parietal pleura is the outer layer and contacts to the thoracic wall, the mediastinum, and the diaphragm. The hilum is the point of connection between the visceral and parietal layers. The space between the parietal and...
7.3K
Gross Anatomy of the Lungs01:17

Gross Anatomy of the Lungs

The lungs are a pair of vital organs connected to the trachea via the left and right bronchi. The base of these organs meets the dome-shaped muscle known as the diaphragm. Encased by the pleurae, the lungs contact the mediastinum. The right lung is shorter yet wider, and has a larger volume than the left lung. The left lung has an indentation known as the cardiac notch. The superior region of the lungs is referred to as the apex, whereas the base is the lower region near the diaphragm. The...
4.7K